建立了固相萃取结合离子色谱测定滴眼液中硼酸及硼砂含量的方法。以甲烷磺酸+甘露醇溶液为淋洗液、四甲基氢氧化铵+甘露醇溶液为再生液,用离子排斥分析柱分离、抑制型电导检测器检测,在浓度范围1~200 μg/mL内硼酸线性关系良好,相关系数r=0.999 6,检出限为0.5 μg/mL。精密度及稳定性良好,低、中、高3种水平回收率分别为102.5%、99.1%、98.5%,RSD分别为1.6%、1.3%、0.8%。该方法快速、准确,为滴眼液中硼酸及硼砂的测定提供了技术支持。

A method is established for determining the contents of boric acid and borax in eye drops by solid phase extraction combined with ion chromatography.Methanesulfonic acid-mannitol solution is used as eluent,while tetramethylammonium hydroxide-mannitol solution is used as regeneration solution,samples are separated by ion exclusion column and detected by suppressed conductivity detector.It is shown from results that boric acid concentration exhibits good linearity in the range of 1-200 μg·mL^-1,with a correlation coefficient of 0.999 6,and a LOD of 0.5 μg·mL^-1.Detection for the contents of boric acid and borax has good precision and stability,the low,medium,high spiked recoveries are 102.5%,99.1%,98.5%,respectively,and RSDs equal to 1.6%,1.3%,0.8%,respectively.This method is rapid and accurate,which can provide technical supports for the determination of boric acid and borax in eye drops.

倪亚萍(1987-),女,硕士生,研究方向为药物制剂及分析,854726191@qq.com