采用星点设计-效应面法优化天然低共熔溶剂(NADESs)提取栀子中绿原酸、栀子苷、西红花苷Ⅰ、西红花苷Ⅱ的工艺,合成了15种NADESs,在单因素实验的基础上优选出最佳提取工艺并进行验证。筛选出氯化胆碱(ChCl)-醋酸为最佳提取溶剂,提取工艺经优化后得到最佳提取条件为:ChCl与醋酸摩尔比为1∶2、含水量45%、固液比(栀子粉末与NADESs的比)1 g∶15 mL、超声时间45 min、超声温度55℃。此条件下绿原酸、栀子苷、西红花苷Ⅰ、西红花苷Ⅱ的提取率分别为1.46、48.44、6.17、0.81 mg/g,总得率为56.88 mg/g,与模型理论值57.21 mg/g的相对误差为0.58%,证明建立的模型能较为准确地预测实验结果。

Central composite design-response surface method is utilized to optimize the extraction of chlorogenic acid,gardenoside,crocin-Ⅰ and crocin-Ⅱ by natural deep eutectic solvent.Fifteen kinds of NADESs are synthesized.On the basis of single factor experiment,the optimal extraction process is obtained and verified by CCD-RSM.Choline chloride-acetic acid is selected as the best solvent.The optimal extraction process conditions are obtained as follows:the molar ratio between choline chloride and acetic acid is 1∶2,water content is 45%,the ratio of Gardenia jasminoides Ellis powder to NADEs is 1∶15,the extraction process spends 45 min and extraction temperature remains at 55℃.Under these optimal conditions,the extraction rates of chlorogenic acid,gardenoside,crocin-Ⅰ and crocin-Ⅱ are 1.46 mg·g^-1,48.44 mg·g^-1,6.17 mg·g^-1 and 0.81 mg·g^-1,respectively.Total yield is 56.88 mg·g^-1,representing a relative error of 0.58% compared with the theoretical value of 57.21 mg·g^-1,which indicates that the established model is reliable.

冯智翱(1996-),女,硕士生,研究方向为药物质量标准的制订和蒙药药效、药代,15124849909@163.com