以聚乙烯醇(PVA)和羧甲基壳聚糖(CMCS)为原料、醛基环糊精(OCD)为交联剂,通过物理-化学交联制备了高强度、血液相容性良好的PVA/CMCS/OCD水凝胶,然后研究了多糖组分含量对水凝胶性能的影响。通过红外光谱仪和扫描电子显微镜表征水凝胶的微观结构,结果表明,PVA和CMCS通过动态亚胺键、氢键和网络缠结形成互穿网络,这不仅减小了水凝胶的孔径,还提高了水凝胶的网络稳定性。同时,力学性能和抗疲劳性能也因为"刚柔相济"的网络结构而得到加强,拉伸模量从0.54 MPa提高到0.76 MPa,性能更贴近天然血管。此外,PVA/CMCS/OCD水凝胶还具有良好的细胞相容性和血液相容性。细胞毒性和溶血率均符合医疗器械生物学评价要求,蛋白质吸附、血小板粘附测试结果表明该水凝胶具有良好的抗凝血性能;复钙化时间测试和动态凝血时间测试结果显示该水凝胶的内源性凝血程度低。因此,成本低廉、制备方法简便、综合性能良好的PVA/CMCS/OCD水凝胶有望用于人工血管。

PVA/CMCS/OCD hydrogel with high-strength and good blood compatible is prepared through physical-chemical cross-linking method using polyvinyl alcohol (PVA) and carboxymethyl chitosan (CMCS) as raw materials,and aldehyde cyclodextrin (OCD) as cross-linking agent.The effect of polysaccharide fraction content on the properties of hydrogel is investigated.The microstructure of the hydrogel is characterized by means of FT-IR and SEM.It is found that PVA and CMCS form interpenetrating networks through dynamic imine bonding,hydrogen bonding,and network entanglement,which reduces the pore size of hydrogel and improves its network stability.Meanwhile,the mechanical properties and fatigue resistance of the hydrogel are also enhanced by the "rigid-flexible" network structure,with the tensile modulus increasing from 0.54 MPa to 0.76 MPa.The performance of the hydrogel is closer to the requirements of natural blood vessels.In addition,PVA/CMCS/OCD hydrogel owns excellent cytocompatibility and hemocompatibility,and its cytotoxicity and hemolysis rates meet the requirements of the biological evaluation of medical devices.The results from protein adsorption and platelet adhesion tests indicate that the hydrogel shows good anticoagulant properties.It is shown through recalcification and dynamic coagulation tests that the hydrogel has a low degree of endogenous coagulation.Therefore,PVA/CMCS/OCD hydrogel with low cost,easy preparation method,and good overall performance is expected to be used for artificial blood vessels.

黄灿锋(1996-),男,硕士生,研究方向为功能水凝胶,1078851487@qq.com