采用光谱法研究了两种降血糖药物氯磺丙脲(CPM)和阿司匹林(ASA)分别与牛血清白蛋白(BSA)的作用机制,以及ASA对CPM与BSA结合的影响。荧光光谱法实验结果表明,ASA、CPM与BSA均形成复合物,其结合常数分别为1.60×10⁴和1.11×10³ mol/L。由热力学参数(ΔH、ΔS和ΔG)可知,ASA与BSA通过范德华力自发结合,而CPM与BSA通过疏水作用自发结合。ASA、CPM主要结合在BSA的SiteⅡ。紫外光谱法实验结果表明,ASA、CPM导致氨基酸残基周围的微环境发生了改变,当ASA存在时,CPM与BSA作用的结合常数减少、结合距离增大。

Spectroscopy method is employed to study the mechanism of chlorpropamide (CPM) and aspirin (ASA) respectively with bovine serum albumin (BSA),and the effect of ASA on the binding of CPM with BSA.It is shown from fluorescence spectroscopy experiment results that both ASA and CPM form complexes with BSA,and the binding constants are 1.60×10⁴ mol·L^-1 and 1.11×10³ mol·L^-1,respectively.It is known from thermodynamic parameters (ΔH,ΔS,ΔG) that ASA and BSA spontaneously bind through Van der Waals forces,while CPM and BSA spontaneously bind through hydrophobicity.ASA and CPM are mainly bound to SiteII of BSA.The results from ultraviolet spectroscopy show that both ASA and CPM change the microenvironment around amino acid residues.In the presence of ASA,the binding constant between CPM and BSA drops,and the binding distance increases.

何茜(1997-),女,硕士研究生,研究方向为分析化学,1040702716@qq.com