以4，7-二溴-2，1，3-苯并噻二唑为原料，设计合成以7-硝基-2，1，3-苯并氧杂GFDA1二唑（NBD）为荧光响应识别基团、4-（7-溴-2，1，3-苯并噻二唑）-苯酚为荧光发射基团的新型H₂S反应型荧光探针（LO-NBD），经核磁共振氢谱（NMR）、红外（IR）、质谱（ESI-MS）和元素分析确证探针结构。紫外-可见吸收光谱和荧光发射光谱测试结果表明，在较宽的pH范围内（6.0~9.0），探针LO-NBD对H₂S表现出高度专一选择性和高灵敏度，具有荧光增强显著、检测限低和实时检测等优点，检测限可低至0.17×10^-6 mol/L。对现有工业废水进行加标回收试验，加标回收率在97.70%~101.75%。

A novel fluorescence probe (LO-NBD) for H₂S detection, with 7-nitro-2, 1, 3-benzoxadiazole moiety (NBD) as response recognition group and 4-(7-bromo-2, 1, 3-benzothiadiazole)-phenol as fluorescent emitter, is synthesized from 4, 7-dibromo-2, 1, 3-benzothidiazole in two steps.The structure of LO-NBD is characterized by NMR, IR, ESI-MS and elemental analysis.It is shown that in a relative broad pH range (from 6.0 to 9.0), LO-NBD probe exhibits high specificity, selectivity and sensitivity to H₂S, with a detection limit as low as 0.17 μM, showing the advantages of significant fluorescence enhancement, low detection limit and real-time detection.The recoveries range from 97.70% to 101.75% in detecting the existing industrial wastewater.