以螺蛳蛋白（Msp）为原料，通过转谷氨酰胺酶（TGase）钙双交联反应制备了大分子质量的酶交联螺蛳蛋白/Ca²⁺絮凝剂（TGase-Msp/Ca²⁺），利用FT-IR、SEM-EDS、Zeta电位对其结构进行表征，考察了TGase-Msp/Ca²⁺对高岭土悬浮液絮凝性能的影响。结果表明，Ca²⁺浓度影响TGase-Msp/Ca²⁺的絮凝性能，在Ca²⁺浓度为0.2 mol/L时TGase-Msp/Ca²⁺因具有良好的桥接作用和电荷中和能力，表现出优秀的絮凝性能并具有较小污泥体积。在TGase-Msp/Ca²⁺用量为1.25 mL、pH为6时，浊度去除率达到99.89%，污泥体积分数为28 mL/L。同时，TGase-Msp/Ca²⁺具有很宽的絮凝范围，在pH 2~12的范围内去除率达到97%以上，絮体粒径达到18 μm，沉降速度为2.21 mm/s。

A large molecular weight enzyme-crosslinked mud snail protein/Ca²⁺ flocculant (TGase-Msp/Ca²⁺) is prepared through double cross-linking with transglutaminase (TGase) and calcium with mud snail protein (Msp) as raw materials, and its structure is characterized by FT-IR, Zeta, SEM-EDS.The influences of TGase-Msp/Ca²⁺ on the flocculation performance of kaolin suspensions are investigated.It is verified that Ca²⁺ content influences the flocculation performance of TGase-Msp/Ca²⁺.As Ca²⁺ content is 0.2 mol·L^-1, TGase-Msp/Ca²⁺ has good bridging and charge neutralization ability, which makes it exhibit excellent flocculation performance and generate a small sludge volume.The removal rate of turbidity reaches 99.89% and the volume fraction of sludge is 28 mL·L^-1 when pH is 6 and the dosage of TGase-Msp/Ca²⁺ is 1.25 mL.In addition, TGase-Msp/Ca²⁺ has a wide flocculation range.The removal rate of turbidity can exceed 97% in the range of pH 2-12.Meanwhile, the floc particle size of TGase-Msp/Ca²⁺ reaches 18 μm with a settling rate of 2.21 mm·L^-1.

毛少辉(1997-),男,硕士研究生,主要从事新型功能材料方面的研究工作,1642567105@qq.com