诺氟沙星（NOR）环境残留会造成一系列生态和环境问题，而高级氧化技术可有效降解废水中的抗生素，但可能产生毒性更强的降解产物。选择秀丽隐杆线虫（C.elegans）作为受试生物，考察NOR氧化降解液对其生理、生化和分子指标的影响，并通过定量结构活性关系预测NOR及其降解产物毒性。结果表明，NOR降解液会刺激线虫运动行为，抑制其生长发育，而对活性氧自由基（ROS）和细胞凋亡无显著影响。然而，线虫氧化应激和神经毒性相关基因表达显著提高，表明造成氧化应激反应，线虫体内ROS被防御机制清除；线虫运动神经受损，进而导致其运动行为异常。毒性预测结果表明，哌嗪环裂解产物是主要毒性物质。

Environmental residues of norfloxacin (NOR) can cause a series of ecological and environmental issues.Although the advanced oxidation technology can effectively degrade antibiotics in wastewater, its degradation products may be more toxic.Caenorhabditis elegans(C.elegans) is chosen as the testing organism, and the toxic effect of NOR degradation solution (NDS) on its physiological, biochemical and molecular indexes is investigated.Meanwhile, the toxicity of NOR and its degradation products is predicted through the quantitative structure-activity relationship method.The results show that NDS can stimulate the locomotion behaviors of C.elegans and inhibit its growth, but does not significantly affect the reactive oxide species (ROS) and apoptosis levels.Oxidative stress and neurotoxicity-related gene expression of C.elegans increases significantly, indicating that C.elegans are caused to generate oxidative stress response, and the defense mechanism of C.elegans is able to eliminate ROS from its body.The motor nerve of C.elegans is damaged, which leads to its abnormal motor behavior.The toxicity prediction results show that the main toxic substances might be originated from the piperazine ring splitting of NOR.

闫莹(1980-),女,博士,副教授,研究方向为环境毒理学,wendy@ecust.edu.cn