从某炼油厂柴油罐区污染土壤中分离筛选出优势柴油降解菌L12，并经过形态学观察、生理生化试验以及16S rDNA序列分析，鉴定为不动杆菌属（Acinetobacter.sp）。采用摇瓶实验研究了菌株L12对正十六烷的适宜降解条件，并考察了该菌对柴油的降解能力。结果表明，菌株L12最佳的培养初始pH为7、接种量为5%、摇床转速为150 r/min、培养温度为30℃，菌株可耐受正十六烷质量浓度高达5 000 mg/L。最优条件下培养7 d，该菌株对正十六烷的降解率高达90.33%，能将质量浓度为3 000 mg/L的柴油几乎完全降解，表明该菌具有良好的用于生物修复柴油污染土壤的潜力。

L12, a dominant diesel-degrading bacterium, is isolated and screened out from the contaminated soil in the diesel tank area of a refinery.After morphological observation, physiological tests, biochemical tests and 16S rDNA sequence analysis, it is identified as Acinetobacter.sp.The shake flask experiment is utilized to study the suitable degradation conditions of L12 strain to n-hexadecane, and the degradation ability of L12 strain to diesel is investigated.The optimal cultural conditions for L12 strain are obtained as follows:the initial pH is 7, the inoculum amount is 5%, the shaking speed is 150 r·min^-1, and the culture temperature is at 30℃.The strain can tolerate the concentration of n-hexadecane up to 5 000 mg·L^-1.If L12 strain has been cultivated for 7 days under the optimal conditions, the strain's degradation rate to n-hexadecane is as high as 90.33%, it can almost completely degrade diesel fuel with a concentration of 3 000 mg·L^-1 within 7 days, indicating that the bacteria has a good potential for bioremediation of diesel polluted soil.

王兴龙(1996-),男,硕士研究生,研究方向为生物修复,724216490@qq.com