通过敲除编码6-葡萄糖磷酸脱氢酶基因zwf和编码磷酸葡萄糖异构酶基因pgi，对合成D-1，2，4-丁三醇（BT）的重组大肠杆菌胞内辅因子NADPH再生进行扰动，考察NADPH/NADP⁺对BT合成的影响。结果显示，pgi基因缺失菌株MJ135kpG胞内NADPH/NADP⁺增加了25%，培养基中合理添加葡萄糖可以增强胞内NADPH供应，促进BT合成。当初始葡萄糖浓度为2 g/L时，24 h时再补加葡萄糖4 g/L可以进一步提高BT产量至4.13 g/L。

D-1, 2, 4-Butanetriol (BT) is an important intermediate in organic synthesis.Cytosolic cofactor NADPH of recombinant Escherichia coli that is used for synthesis of BTO is disturbed by deletion of zwf encoding for 6-glucose phosphate dehydrogenase and pgi encoding for glucose isomerase, to investigate the effects of NADPH/NADP⁺ on BTO synthesis via recombinant Escherichia coli.The results show that cytosolic NADPH/NADP⁺ in MJ135kpG strain with pgi gene deleted increases by 25%, and the appropriate addition of glucose in the medium can enhance the supply of cytosolic NADPH, which can promote the synthesis of BTO.As the initial concentration of glucose is 2 g·L^-1, the yield of BTO reaches 4.02 g·L^-1 in shake flask, and further increases to 4.13 g·L^-1 by adding glucose 4 g·L^-1 at 24 h.In a sum, enhancing NADPH supply is an effective strategy to improve BTO yield.

谷松鹤(1995-),女,硕士生,研究方向为生物化学与分子生物学,468304203@qq.com