通过将11-巯基-十一烷酸自组装到金电极表面、利用EDC/NHS活化羧基促进凝集素分子（ConA）的固载，构建了用于检测一种具有良好缓蚀性能的海洋细菌（S-59）的生物传感器。通过电化学阻抗谱探究了S-59在Q235碳钢表面的电化学行为，并利用ConA修饰金电极传感器系统对不同浓度菌液进行检测，建立了电荷转移电阻（R_ct）与S-59浓度之间的线性关系，线性检测范围为10³~10⁸ cfu/mL，同时验证了该传感器具有较好的特异性、稳定性和重复性。

An electrochemical sensor for the detection of marine bacteria (S-59) with good corrosion inhibition performance is constructed through self-assembling 11-mercaptoundecanoic acid onto the surface of gold electrode and promoting the immobilization of lectin-Concanavalin A (ConA) by activating carboxyl group with EDC/NHS.The corrosion inhibitory effect of S-59 on Q235 carbon steel surface is investigated by means of electrochemical impedance spectroscopy (EIS).ConA modified gold electrode sensor system is applied to detect different concentrations of bacterial solution.A linear relationship between charge transfer resistance (R_ct) and S-59 concentration is established.The linear detection ranges from 10³ cfu·mL^-1 to 10⁸ cfu·mL^-1.Good specificity,stability and repeatability of the sensor are also verified.

王治文(1994-),男,本科生