通过比较7种不同来源的产脂肪酶菌种催化性能，选用性能较好的Rhizopus oryzae CGMCC 3.5040细胞催化α-蒎烯的环氧化反应合成2，3-环氧蒎烷，利用单因素试验法研究了反应过程中反应时间、H₂O₂体积分数、细胞质量、束酸剂物质的量、辛酸浓度对反应的影响，随后采用正交试验法优化工艺条件。结果表明，最优的工艺反应条件为：反应时间为12 h、双氧水与蒎烯摩尔比为7.5∶1、细胞质量为350 mg、束酸剂物质的量为3.5 mmol、辛酸浓度为1 mol/L，此条件下Rhizopus oryzae CGMCC 3.5040细胞催化α-蒎烯环氧化反应的转化率为（97.9±0.9）%，收率为（85.4±1.1）%。

Through comparing the catalytic performances of seven lipase producing strains from different sources, Rhizopus oryzae CGMCC 3.5040 cells with better performance is screened out and used to catalyze the epoxidation of α-pinene to synthesize α-pinene oxide. Effects of reaction time, H₂O₂ dosage, cell dosage, acid trapping agent dosage and octanoic acid concentration on the reaction are studied by means of single factor test. The process conditions are then optimized via orthogonal test method. The optimal reaction conditions are obtained as follows:reaction time is 12 h, the molar ratio of hydrogen peroxide to pinene is 7.5∶1, the cell dosage is 350 mg, the dosage of acid trapping agent is 3.5 mmol, and the concentration of octanoic acid is 1 mol·L^-1. Under the optimal conditions, the conversion rate of α-pinene epoxidation reaches (97.9±0.9)%, and the yield of α-pinene oxide reaches (85.4±1.1)%.

涂林(1995-),男,硕士研究生,主要从事生物催化合成方面的研究,634606736@qq.com