利用自组装技术，在玻碳电极（GCE）表面构建了一种基于聚乙烯亚胺（PEI）/手性肽（D-BGAc）的手性界面，用于对色氨酸（Trp）对映体的电化学识别。首先在玻碳电极表面修饰一层PEI，D-BGAc与PEI通过静电自组装形成多孔膜，制备得到PEI/D-BGAc手性界面，并对其进行了表征。差分脉冲伏安法（DPV）的分析表明，在最优条件下，此手性界面对于L-Trp和D-Trp之间的电流识别效果（I_D/I_L）可达3.4。在0.005~0.15 mmol/L的浓度范围内，不同浓度的Trp对映体与其峰电流有良好的线性关系。此外，手性界面还能够用于识别Trp外消旋溶液，显示该手性界面能成功用于Trp对映体的识别。

A chiral interface based on polyethyleneimine (PEI) and chiral peptide (D-BGAc) is constructed on the surface of glassy carbon electrodes (GCE) by self-assembly technology and used for electrochemical recognition of tryptophan (Trp) enantiomers.Firstly,a layer of PEI is modified onto the surface of a glassy carbon electrode.PEI/D-BGAc chiral interface is manufactured by electrostatic self-assembly of D-BGAc and PEI,and is characterized.Differential pulse voltammetry (DPV) results show that the current recognition effect (I_D/I_L) between L-tryptophan (L-Trp) and D-tryptophan (D-Trp) can reach 3.4 by PEI/D-BGAc chiral interface under the optimal conditions.In addition,within the concentration range of 0.005 mM to 0.15 mM,different concentrations of Trp enantiomers show a better linear relationship with the corresponding peak currents.More importantly,the chiral interface can also be used to identify racemic Trp solutions,i.e.to quickly determine the relative content of either enantiomer.Therefore,the chiral interface can be successfully used for the recognition of electrochemical enantiomers.

何家辉(1995-),男,硕士生,研究方向为生物材料,861096281@qq.com