以商业固定化脂肪酶复配的组合酶（米黑毛霉脂肪酶+南极假丝酵母脂肪酶）为催化剂、栅藻藻油为原料制备生物柴油，对反应体系和条件进行全面优化，得出最理想的酶催化反应体系和条件分别为：藻油与甲醇摩尔比为1：12、助溶剂（叔丁醇）：藻油为1：1（V/m）、藻油与固定化脂肪酶质量比为50：1、反应温度为45℃、反应时间为9~12 h、摇床转速为250 r/min。在此条件下，制得的生物柴油（FAME）最高转化率达98.67%，为微藻生物柴油的工业化开发和应用提供了参考依据。

Biodiesel is prepared by using Scenedesmus oil as raw materials and the combinatorial enzymes compounded by commercial immobilized lipase (Mucor miehei lipase combined Candida antarctica lipase) as catalyst,and the reactive system and conditions are systematically optimized.The optimum reaction system and conditions are obtained as follows:the molar ratio between algae oil and methanol is 1∶12,the ratio of co-solvent (t-butanol) to algae oil is 1∶1(v/m),the mass ratio of algae oil to immobilized lipase is 50∶1,reaction temperature maintains at 45℃,reaction lasts for 9-12 h,shaking speed is at 250 r·min^-1.Under these conditions,high-purity biodiesel (FAME) can be produced with a maximum conversion rate of 98.67%.Results from this study will provide an important theoretical basis for the development and application of microalgae-based biodiesel.

张桐栋(1998-),男,本科,研究方向为固定化酶催化反应,zhang＿td＿academic@163.com