现代化工

现代化工 ›› 2020, Vol. 40 ›› Issue (8) : 218-220,226. DOI: 10.16606/j.cnki.issn0253-4320.2020.08.048

分析测试

二氧化锰纳米颗粒比色法测定谷胱甘肽

李玲芳, 尹俊霞, 贾桥, 马翔, 王琦

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Determination of glutathione by manganese dioxide nanoparticles based colorimetry

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Received		Published
2019-10-30		2020-08-20
Issue Date	Revised Date
2020-08-14	2019-10-30

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摘要

基于二氧化锰纳米颗粒具有优异的模拟酶特性，能够高效地催化氧化3，3'，5，5'-四甲基联苯胺产生明显的蓝色变化，而谷胱甘肽的加入能够引起二氧化锰的降解从而抑制了基于二氧化锰的显色反应。据此制备了二氧化锰纳米颗粒并且建立了测定谷胱甘肽的比色法，结果表明，在最佳条件下，谷胱甘肽的线性范围为8~70 μmol/L，并且表现出了良好的选择性。

Abstract

Manganese dioxide nanoparticles (MnO₂ NPs) reveal excellent mimetic enzyme performances,and can efficiently catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to generate an obvious blue change.However,the addition of glutathione (GSH) can induce the decomposition of MnO₂ and thus inhibit the color reaction based on MnO₂.Therefore,MnO₂ NPs are synthesized and a colorimetry method for GSH determination is established.It is turned out that under the optimal conditions,the linear range of glutathione is 8-70 μmol·L^-1 with a good selectivity.