采用自组装方法构建金磁微粒（Fe₃O₄@Au）复合纳米粒子，并表征其理化性能。评估Fe₃O₄@Au模拟酶的催化动力学过程，并基于其过氧化物模拟酶活性，优化出具有高灵敏度和选择性的检测过氧化氢体系。研究结果表明，Fe₃O₄@Au相比于天然过氧化物酶具有更小的K_m数值；H₂O₂最佳检测条件为Fe₃O₄@Au浓度0.075 mg/mL、催化时间60 min和催化温度70℃；在最适检测体系下，H₂O₂浓度在0.005~0.08 mol/L时，吸光度数值与其具有良好线性关系，工作曲线方程为y=22.966x+0.689，检出限为4.9 μmol/L。

Fe₃O₄@Au nanomaterials are prepared by self-assembly method,and their physical and chemical properties are characterized.The catalytic kinetic process of Fe₃O₄@Au mimetic enzyme is evaluated.Based on its peroxidase mimetic enzyme activity,a highly sensitive and selective detection system for hydrogen peroxide is optimized.The results show that the prepared Fe₃O₄@Au has smaller K_m compared with natural peroxidase.The optimum conditions for detection of hydrogen peroxide are as follows:Fe₃O₄@Au concentration is 0.075 mg·mL^-1,catalytic time is 60 min and catalytic temperature is kept at 70℃.Under the optimal detection system,the working curve equation of hydrogen peroxide is y=22.966x+0.689 when H₂O₂ concentration is in the range of 0.005-0.08 mol·L^-1,and the detection limit is 4.9 μmol·L^-1.