以煤为原料，通过回流法制备具有强烈荧光的煤基碳点。铅离子能使碳点荧光显著猝灭，体系荧光信号"关闭"；加入多巴胺后，能使碳点荧光得以恢复，体系荧光信号处于"打开"状态。据此建立碳点"关-开"型荧光探针检测多巴胺的新方法。在优化实验条件下，碳点荧光恢复强度与多巴胺浓度在0.5~25.0 μmol/L范围内呈良好线性关系，检出限（3S_b/S）为 0.1 μmol/L。该方法用于样品中多巴胺的测定，加标回收率为96.5%~104.5%，相对标准偏差（RSD）为1.8%~2.7%。

Carbon-dots with strong fluorescence are prepared through reflux method with coal as precursors.Lead ions can enable the fluorescence of carbon-dots to quench significantly,and therefore the fluorescence signal of the system is in the "off" state.The addition of dopamine can make the fluorescence of carbon-dots recover,and the fluorescence signal of the system is in the "on" state.Hence,a new method for the determination of dopamine is built based on coal-based carbon-dots "off-on" fluorescence probe.Under optimal conditions,the recovery intensity of carbon-dots fluorescence shows good linear relationship to the concentration of dopamine in the range of 0.5-25.0 μmol·L^-1,with a detection limit (3S_b/S) of 0.1 μmol·L^-1.As this method is applied for the determination of dopamine in real samples,the spiked recovery is in the range of 96.5%-104.5% and the relative standard deviation (RSD) is in the range of 1.8%-2.7%.

毛永强(1974-),男,博士,副教授,研究方向为功能纳米材料,maoyq@tju.edu.cn