在改性SiO₂表面接枝聚合GMA-ID-Cu²⁺后，再与温敏单体（NIPAM）共聚合成温敏型分子印迹聚合物（MIP），并利用GMA-IDA-Cu²⁺金属螯合作用对模板蛋白BSA进行选择性吸附。通过TEM、IR和TGA测试表明，印迹层已接枝在SiO₂外层，且印迹层厚度约9 nm。pH和温度条件的优化试验表明，MIP具有优异的温度敏感性，并在pH为4.6时获得较高的吸附容量（115.42 mg/g）和印迹因子（2.58）。对不同蛋白质的特异性吸附试验说明，MIP内部的印迹空腔和印迹位点与模板蛋白BSA相互匹配。同时MIP具有较高的吸附速率和较好的重复性。

A thermo-sensitive molecularly imprinted polymer (MIP) is synthesized through graft copolymerization of "whisker" polymer chains on the surface of modified SiO₂ and then copolymerization with thermo-sensitive monomers (NIPAM).The template protein bovine serum albumin (BSA) is selectively adsorbed by GMA-IDA-Cu²⁺ by means of metal chelation.TEM,IR and TGA measurements prove that the imprinted layer has been grafted onto SiO₂ outer layer,and the thickness of the imprinted layer is around 9 nm.The optimum experiments for pH and thermo-sensitive conditions verify that MIP has an excellent temperature sensitivity.An adsorption capacity as high as 115.42 mg·g^-1 and an imprinting factor of 2.58) are obtained at pH 4.6.Specific adsorption tests for different proteins show that the imprinted cavities and imprinted sites inside the MIP match well with the template protein BSA.In addition,the MIP also exhibits higher adsorption rate and better repeatability.

董祥芝(1984-),女,硕士,工程师,研究方向为环境响应型蛋白质分子印迹材料的合成与性能研究,dongxz6566@foxmail.com