从油污土壤中筛选出一株高效降解噻吩的微生物，对其进行分类鉴定和培养条件优化。经16S rDNA基因序列分析鉴定，该菌株为肠杆菌属（Enterobacter sp.）。建立了高效液相色谱法检测液体中噻吩的方法。优化后的培养条件为：温度为30℃、初始pH 8.0。培养基组成为：葡萄糖0.6 g/L、磷酸氢二钾2.5 g/L、氯化镁0.2 g/L、氯化钠0.24 g/L、磷酸氢二铵0.3 g/L。在0.1 g/L噻吩质量浓度下培养24 h，菌体对噻吩的降解率达到了66.26%，表明该菌株具有潜在的可用于处理含噻吩汽油的能力。

A bacterial strain capable of degrading thiophene is screened from oil-contaminated soil,and then is classified and identified.Its culture conditions are optimized too.This strain is identified as Enterobacter sp.via the 16S rDNA gene sequence analysis.A method for detecting thiophene in liquid by high performance liquid chromatography is established.The optimized culture conditions include that the initial pH is 8.0 and temperature is 30℃.The optimized culture medium is composed of 0.6 g·L^-1 glucose,2.5 g·L^-1 dipotassium hydrogen phosphate,0.2 g·L^-1 magnesium chloride,0.24 g·L^-1 sodium chloride and 0.3 g·L^-1 diammonia hydrogen phosphate.The degradation efficiency of thiophene reaches 66.26% after it has been cultured under 0.1 g·L^-1 of thiophene for 24 h.It is demonstrated that this strain has the potential ability to treat with thiophene-containing gasoline.