Abstract: Six strains of microalgae are isolated and purified from the nature.Among them,an excellent oil producing strain is obtained through screening and cultivating microalgae strains in a 200 mL column photobioreactor.Experimental results show that the total liquid contents and dry weights of six strains of microalgae all exceed 20% and 1.2 g·L-1,respectively.Among them,the strain SS6 grows the fastest and enters stable phase after 12 days in the photobioreactor.Its OD689,biomass,grease contents and grease productivity reach 12.270 3,1.337 g·L-1,30.1% and 25.9 mg/(L·d),respectively.Phylogenetic analysis by 18S rDNA identifies that SS6 is Monoraphidium komarkovae.By evaluating various preservation methods,it is found that SS6 can be stored for more than one year at -18℃ under the protection by glycerol (20% v/v).After recovery,its abilities in growth and oil production both can maintain the states before preservation.
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