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现代化工  2019, Vol. 39 Issue (7): 220-223    DOI: 10.16606/j.cnki.issn0253-4320.2019.07.047
  分析测试 本期目录 | 过刊浏览 | 高级检索 |
基于紫外-可见吸收光谱法检测药物中地奥司明
庞向东, 成晓琼, 刘贵秀, 江虹
长江师范学院化学化工学院, 长江师范学院武陵山片区绿色发展协同创新中心, 重庆 408100
Determination of diosmin in drugs by ultraviolet-visible absorption spectrometry
PANG Xiang-dong, CHENG Xiao-qiong, LIU Gui-xiu, JIANG Hong
Collaborative Innovation Center for Green Development in Wuling Mountain Areas, School of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China
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摘要 在pH 6.70的弱酸性溶液中,乙基紫与地奥司明反应生成一种二元配合物,在紫外光区345 nm处产生1个较强的正吸收峰,在可见光区594 nm处产生1个较强的负吸收峰,在此二波长处,地奥司明的质量浓度在0.3~15.2 mg/L范围内与吸光度的绝对值(|A|)呈线性关系并服从朗伯-比尔定律,其表观摩尔吸光系数(κ)为1.88×104 L/(mol·cm)(正吸收)和3.81×104 L/(mol·cm)(负吸收),检出限为0.24 mg/L (正吸收)和0.18 mg/L (负吸收)。当以负吸收为参比波长、正吸收为测定波长、用双波长法测定时,其线性范围不变,表观摩尔吸光系数(κ)为5.70×104 L/(mol·cm),检出限为0.11 mg/L。由此建立了测定地奥司明的紫外-可见吸收光谱法。该方法可用于市售地奥司明药物中地奥司明的测定。
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庞向东
成晓琼
刘贵秀
江虹
关键词:  吸收光谱法  地奥司明  乙基紫    
Abstract: In a weak acidic solution with a pH of 6.70,ethyl violet can react with diosmin to form a binary complex,resulting in that a strong positive absorption peak is generated at 345 nm in the ultraviolet region,and a strong negative absorption peak is igenerated at 594 nm in the visible region.At these two wavelengths,the mass concentration of diosmin is linearly related to the absolute value of absorbance (|A|) in the range of 0.3 to 15.2 mg/L,which obeys lambert-beer's law.Their apparent molar absorption coefficients (κ) are 1.88×104 L/(mol·cm) (positive absorption) and 3.81×104 L/(mol·cm) (negative absorption) respectively.Their detection limits are 0.24 mg/L (positive absorption) and 0.18 mg/L (negative absorption).If the negative absorption wavelength is used as reference wavelength,the positive absorption wavelength is used as determination wavelength and a dual-wavelength absorption spectrometry is used to determine diosmin,its linear range remains unchanged,its apparent molar absorption coefficient (κ) is 5.70×104 L/(mol·cm) and its detection limit is 0.11 mg/L.Based on this,the ultraviolet-visible absorption spectrometry is established for the determination of diosmin.The method can be applied to the determination of diosmin in commercially available diosmin drugs with the satisfactory results.
Key words:  absorption spectrometry    diosmin    ethyl violet
收稿日期:  2018-10-28      修回日期:  2019-05-07           出版日期:  2019-07-20
O657.3  
  R917  
基金资助: 重庆市教委科技基金资助项目(KJ1401226);长江师范学院科技基金资助项目(2018CXX150)
通讯作者:  江虹(1956-),女,理学学士,教授,研究方向为分子光谱分析,通讯联系人,jianghongch@163.com。    E-mail:  jianghongch@163.com
作者简介:  庞向东(1962-),男,理学学士,副教授,研究方向为分子光谱分析,cspxdfxsch123456@163.com
引用本文:    
庞向东, 成晓琼, 刘贵秀, 江虹. 基于紫外-可见吸收光谱法检测药物中地奥司明[J]. 现代化工, 2019, 39(7): 220-223.
PANG Xiang-dong, CHENG Xiao-qiong, LIU Gui-xiu, JIANG Hong. Determination of diosmin in drugs by ultraviolet-visible absorption spectrometry. Modern Chemical Industry, 2019, 39(7): 220-223.
链接本文:  
http://www.xdhg.com.cn/CN/10.16606/j.cnki.issn0253-4320.2019.07.047  或          http://www.xdhg.com.cn/CN/Y2019/V39/I7/220
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