Determination of diosmin in drugs by ultraviolet-visible absorption spectrometry
PANG Xiang-dong, CHENG Xiao-qiong, LIU Gui-xiu, JIANG Hong
Collaborative Innovation Center for Green Development in Wuling Mountain Areas, School of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China
Abstract: In a weak acidic solution with a pH of 6.70,ethyl violet can react with diosmin to form a binary complex,resulting in that a strong positive absorption peak is generated at 345 nm in the ultraviolet region,and a strong negative absorption peak is igenerated at 594 nm in the visible region.At these two wavelengths,the mass concentration of diosmin is linearly related to the absolute value of absorbance (|A|) in the range of 0.3 to 15.2 mg/L,which obeys lambert-beer's law.Their apparent molar absorption coefficients (κ) are 1.88×104 L/(mol·cm) (positive absorption) and 3.81×104 L/(mol·cm) (negative absorption) respectively.Their detection limits are 0.24 mg/L (positive absorption) and 0.18 mg/L (negative absorption).If the negative absorption wavelength is used as reference wavelength,the positive absorption wavelength is used as determination wavelength and a dual-wavelength absorption spectrometry is used to determine diosmin,its linear range remains unchanged,its apparent molar absorption coefficient (κ) is 5.70×104 L/(mol·cm) and its detection limit is 0.11 mg/L.Based on this,the ultraviolet-visible absorption spectrometry is established for the determination of diosmin.The method can be applied to the determination of diosmin in commercially available diosmin drugs with the satisfactory results.
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PANG Xiang-dong, CHENG Xiao-qiong, LIU Gui-xiu, JIANG Hong. Determination of diosmin in drugs by ultraviolet-visible absorption spectrometry. Modern Chemical Industry, 2019, 39(7): 220-223.
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