Rapid determination of 16 fluoroquinolones in environmental water by high performance liquid chromatography and their verification by mass spectrometry
LI Yu-jing1,2, CHEN Zhi-ran1,2, WANG Xue-ping1,2
1. Yellow River Conservancy Technical Institute, Kaifeng 475004, China; 2. Key Laboratory of Green Coating Materials of Kaifeng City, Kaifeng 475004, China
Abstract: HLB solid-phase extraction column is used to enrich and purify 16 kinds of fluoroquinolones (FQs) in environmental waters.After detection by a variable wavelength scanning ultraviolet detector, the method for determination of remindering FQs in environmental water by high performance liquid chromatography are established.Suspected FQs residual positive water samples are confirmed by liquid chromatography-mass spectrometry.It is found that in the range of 5.0-100.0 μg/mL, the concentrations of 16 kinds of FQs all have a good linear relationship with their peak areas, with correlation coefficient ranging from 0.998 9 to 0.999 9, detection limit ranging from 0.9 μg/mL to 7.3 μg/mL, quantitation limit ranging from 3.0 μg/mL to 24.3 μg/mL, average recoveries ranging from 89.3% to 98.6% and the relative standard deviations ranging from 0.9% to 3.1%.
李玉静, 陈志冉, 王雪平. 高效液相色谱法快速筛查环境水体中16种氟喹诺酮类抗生素及质谱确证[J]. 现代化工, 2018, 38(9): 237-241.
LI Yu-jing, CHEN Zhi-ran, WANG Xue-ping. Rapid determination of 16 fluoroquinolones in environmental water by high performance liquid chromatography and their verification by mass spectrometry. Modern Chemical Industry, 2018, 38(9): 237-241.
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