Please wait a minute...
 
最新公告: 重要提醒:骗子冒充编辑部要求加作者微信,谨防上当!   关于暑假、寒假期间版面费发票及期刊样刊延迟邮寄的通知    
现代化工  2017, Vol. 37 Issue (9): 203-205    DOI: 10.16606/j.cnki.issn0253-4320.2017.09.048
  分析测试 本期目录 | 过刊浏览 | 高级检索 |
蛋白质保护的金纳米簇为荧光探针检测甲磺酸帕珠沙星
胥彦琪
江西医学高等专科学校药学系, 江西 上饶 334000
Detection of pazufloxacin mesylate by protein-protected gold nanoclusters
XU Yan-qi
School of Biological Pharmaceutical Technology, Jiangxi Medical College, Shangrao 334000, China
下载:  PDF (1394KB) 
输出:  BibTeX | EndNote (RIS)      
摘要 基于牛血清白蛋白(BSA)保护的金纳米簇为荧光探针,通过甲磺酸帕珠沙星(PZFX)对其荧光的淬灭实现对PZFX的高灵敏度以及高选择性检测,检测限达2.9×10-8 g/mL。并将此方法用于人尿液样品中PZFX检测,检测结果表明,该方法具有简单、高效、灵敏度高和实用性强等特点,具有较好的应用前景。
服务
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章
胥彦琪
关键词:  基于牛血清白蛋白  金纳米簇  甲磺酸帕珠沙星  尿液样品    
Abstract: Gold nanoclusters protected by bovine serum albumin is used as fluorescent probe,pazufloxacin mesylate (PZFX) is detected with high sensitivity and high selectivity through PZFX's quenching against fluorescent.The detection limit reaches 2.9×10-8 g·mL-1.This method is also used to detect urine samples.The results indicate that this method has good application prospect with characters of simple,efficient,high sensitivity and strong practicability.
Key words:  bovine serum albumin    gold nanoclusters    pazufloxacin mesylate    urine samples
收稿日期:  2017-03-04                出版日期:  2017-09-20
O655  
作者简介:  胥彦琪(1984-),男,硕士,讲师,研究方向为生物制药技术,airln@qq.com
引用本文:    
胥彦琪. 蛋白质保护的金纳米簇为荧光探针检测甲磺酸帕珠沙星[J]. 现代化工, 2017, 37(9): 203-205.
XU Yan-qi. Detection of pazufloxacin mesylate by protein-protected gold nanoclusters. Modern Chemical Industry, 2017, 37(9): 203-205.
链接本文:  
http://www.xdhg.com.cn/CN/10.16606/j.cnki.issn0253-4320.2017.09.048  或          http://www.xdhg.com.cn/CN/Y2017/V37/I9/203
[1] Carlucci G.Analysis of fluoroquinolones in biological fluids by high-performance liquid chromatography[J].Journal of Chromatography A,1998,812(1/2):343-369.
[2] 钟倩.抗菌药甲磺酸帕珠沙星[J].世界临床药物,2004,25(2):122-123.
[3] Phapale B P,Lee H W,Kim S D,et al.Analysis of pazufloxacin mesilate in human plasma and urine by LC with fluorescence and UV detection,and its application to pharmacokinetic study[J].Chromatographia,2010,71:101-106.
[4] Li Q,Wang R,Pei F.Determination of pazufloxacin mesilate in human plasma and urine by high-performance liquid chromatography[J].Asian Journal of Drug Metabolism and Pharmacokinetics,2004,4(4):289-293.
[5] Zhang Z L,Li J J,Qu L B,et al.Determination of pazufloxacin mesylas by capillary electrophoresis with electrochemiluminescence detection[J].Chinese Journal of Analytical Chemistry,2008,36(7):941-946.
[6] Wang Y,Baeyens W R C,HUANG C,et al.Enhanced separation of seven quinolones by capillary electrophoresiswith silica nanoparticles as additive[J].Talanta,2009,77:1667-1674.
[7] Chen S L,Ma H W,Zhao H C,et al.Terbium-sensitized fluorescence method for the determination of pazufloxacin mesilate and its application[J].Analytical Sciences,2004,20:1075-1078.
[8] Wang X L,Chen S L,Zhao H C,et al.Europium sensitized chemiluminescence determination of pazufloxacin mesylatein urine and serum[J].Analytical Letters,2005,38(6):971-979.
[9] Yang C Y,Zhang Z J,Chen S,et al.Molecularly imprinted on-line solid-phase extraction combined with chemiluminescence for the determination of pazufloxacin mesilate[J].Microchimica Acta,2007,159:299-304.
[10] Xie J P,Zheng Y G,Ying J Y.Protein-directed synthesis of highly fluorescent gold nanoclusters[J].Journal of the American Chemical Society,2009,131(3):888-889.
[10] Xie J P,Zheng Y G,Ying J Y.Protein-directed synthesis of highly fluorescent gold nanoclusters[J].Journal of the American Chemical Society,2009,131(3):888-889.
[11] Kawasak H,Yoshimura K,Hamaguchi K,et al.Trypsin-stabilized fluorescent gold nanocluster for sensitive and selective Hg2+ detection[J].Analytical Sciences,2011,27(6):591-596.
[12] Aquila A,Murray R W.Monolayer-protected clusters with fluorescent dansyl ligands[J].Langmuir,2000,16:5949-5954.
[13] Guével X L,Daum N,Schneider M.Synthesis and characterization of human transferrin-stabilized gold nanoclusters[J].Nanotechnology,2011,22(27):275103.
[14] Xavier P L,Chaudhari K,Verma P K,et al.Luminescent quantum clusters of gold in transferrin family protein,lactoferrin exhibiting FRET[J].Nanoscale,2010,2(12):2769-2776.
[15] Kawasak H,Hamaguchi K,Osaka I,et al.pH-dependent synthesis of pepsin-mediated gold nanoclusters with blue green and red fluorescent emission[J].Advanced Functional Materials,2011,21(18):3508-3515.
[16] Zhang M,Dang Y Q,Li H W,et al.Pressure-induced fluorescence enhancement of the BSA-protected gold nanoclusters and the corresponding conformational changes of protein[J].The Journal of Physical Chemistry,2013,117(1):639-647.
[17] Jin J,Zhang X.Spectrophotometric studies on the interaction between pazufloxacin mesilate and human serum albumin or lysozyme[J].Journal of Luminescence,2008,128:81-86.
[1] 吴红梅, 肖昱, 郭宇, 李景阳, 苗世举, 赵亮. 氨基改性SBA-15介孔分子筛的制备及其铬离子吸附性能[J]. 现代化工, 2018, 38(6): 78-82.
[2] 周兴, 孙四娟, 霍登平, 王维佳. 微波消解-ICP-AES法测定电镀金层中的杂质元素含量[J]. 现代化工, 2014, 34(7): 163-166.
[3] 武明丽. 快速EDTA滴定法测定高铅渣料中铅[J]. 现代化工, 2015, 35(7): 171-172,174.
[1] . [J]. Modern Chemical Industry, 2015, 35(11): 77 -80 .
[2] . [J]. Modern Chemical Industry, 2015, 35(12): 128 -130,132 .
[3] . [J]. Modern Chemical Industry, 2017, 37(6): 103 -0106,108 .
[4] . [J]. , 2003, 23(5): 0 .
[5] . [J]. , 2009, 29(6): 0 .
[6] . [J]. , 2010, 30(3): 0 .
[7] . [J]. , 2010, 30(7): 0 .
[8] . [J]. , 2007, 27(2): 0 .
[9] . [J]. Modern Chemical Industry, 2014, 34(2): 131 -133 .
[10] . [J]. Modern Chemical Industry, 2014, 34(4): 14 -16 .
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
京ICP备09035943号-37
版权所有 © 《现代化工》编辑部
本系统由北京玛格泰克科技发展有限公司设计开发 技术支持:support@magtech.com.cn